assay characteristics performance brca hereditary cancer mastr plus the brca hereditary cancer (hc) mastr plus in combination with the mastr reporter brca?hc mastr plus application, is a complete research solution (ngs library?preparation assay, analysis tool and quality control), for the identification of variants in 25 genes? and the 3utr of epcam associated with hereditary breast, ovarian and other related cancers. research application ? for the detection of variants (snvs, small indels) and cnvs, in 26 (25 genes plus the 3utr of epcam ) cancer predisposition associated genes on blood-derived dna using illumina miseq genes brca1, brca2, palb2, chek2, bard1, brip1, rad51c, rad51d, tp53, mre11a, rad50, nbn, fam175a, atm, stk11, men1, pten, cdh1, mutyh, blm, xrcc2, mlh1, msh6, pms2 and msh2, and the 3 utr of epcam genomic region analyzed 140.5 kb number of amplicons 561 amplicon length 232-430 bp number of plexes 5 verifed with ngs system miseq v3 designed to be compatible with miseq compatible analysis options* mastr reporter brca hc mastr plus application *also compatible with analysis tools sophia ddm and jsi seqnext uniformity of amplifcation (0.2x mean coverage) 96.7 % on target read count 98.6 % dna input as low as 20 ng per plex number of samples/run illumina miseq v3: 93 disease-associated genes pten cdh1 fam175a men1 pancreas parathyroid glands kidneys endometrial glands colon stomach bard1 brca1 brca2 brip1 rad51c rad51d tp53 mre11a rad50 nbn breasts ovarians atm palb2 stk11 mutyh chek2 blm xrcc2 epcam* pms2 msh2 msh6 mlh1 *3'utr of epcam *3 utr of epcam only
? agilent technologies, inc. 2017 printed in belgium, december 31, 2017 5991-8379enn pr7000-1404 order info for research use only. not for use in diagnostic procedures. multiplicom n.v. [ a ] galile?laan 18, b-2845 niel, belgium [ w ] www.agilent.com [ e ] mtp.customerservice@agilent.com [ t ] +32 3 289 14 00 [ f ] +32 3 400 23 16 cat. no. product name samples mr-1320.024 brca hereditary cancer mastr plus + mastr reporter brca hereditary cancer mastr plus 24 mid (molecular identifiers) kits are necessary to complete the workflow. workflow automated analysis mastr plus 7h, 2.5h hot ngs ngs sequencer upload a new run variant calling quality control quality parameter context value value graphical indication of value read pile-up views approve sample create analysis report targeted analysis time/sample 40 min* * determined by number of reads in fle if cnv analysis was selected workflow plan run multiplex pcr quality control universal pcr quality control equimolar pooling mixing plexes of single sample puri? cation puri? cation multiplex pcr dilution universal pcr clean up mixing clean up pooling sequencing data analysis mastr plus work? ow ngs
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